By Sidney P. Colowick, Nathan Oram Kaplan, John Abelson, Melvin I. Simon
In recent times, cyanobacteria became the point of interest of excessive research. As uncomplicated micro organism, they might appear to be the most obvious organisms of selection for the examine of such primary tactics as oxygen-evolving photosynthesis and nitrogen fixation. This publication will end up invaluable to investigators with little past adventure with cyanobacteria in addition to to people who desire to swap the emphasis in their learn.
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Often the ecological niche inhabited by the organisms was also of important discriminatory value. Type materials under the 33 M. Herdman, personal communication. 34 p. Bourelly, in "Les Algues d'eau Douce: 3. Les Algues Bleues et Rouges. " Boub6e, Paris, 1970. 35 R. Y. Stanier, in "Bergey's Manual of Determinative Bacteriology" (R. E. Buchanan and N. E. ), p. 22. Williams & Wilkins, Baltimore, Maryland, 1974. 34 ISOLATION, IDENTIFICATION, AND CULTURING  Botanical Code are dried herbarium specimens, descriptions, and illustrations; laboratory cultures are not yet recognized for this purpose, but an attempt to change this has recently been m a d e .
Aquat. Sci. 214, in press (1987). 44 C. Van Baalen, J. Phycol. 1, 19 (1965). 45 T. Vaara, M. Vaara, and S. Niemel~i, Appl. Environ. Microbiol. 38, 1011 (1979). 16 ISOLATION, IDENTIFICATION, AND CULTURING  if used for the solidification of poorly buffered media such as B G l l . 0), particularly if the strains do not tolerate Tris or phosphates at concentrations which permit a good buffering capacity. However, the addition of filter-sterilized NaHCO3 (10 mM), prior to pouring plates, often also overcomes growth problems18; it is not yet known whether this results from an increase of the pH or from the provision of COz in a readily assimilated form ( N a H C O 3 ) , 47 o r from both.
Allen and D. I. Arnon, Plant Physiol. 30, 366 (1955). d R. Rippka, J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier, J. Gen. Microbiol. U l , 1 (1979). centrations of Na +, Mg 2+, Ca 2+, and CI-. 9 The success of medium ASP-2 for non-N2-fixing cyanobacteria 5,37 is to some extent surprising (as is the freshwater medium ASM-1, see Tables I and II), since they both lack molybdenum which is known to be essential for growth on nitrate, 23,24the only source of combined nitrogen supplied, and can only be explained if sufficient quantities of this element are introduced as impurities with the major salts.