Current Protocols in Cytometry by Robinson

By Robinson

Show description

Read Online or Download Current Protocols in Cytometry PDF

Best history & philosophy books

The Lord of Uraniborg: A Biography of Tycho Brahe

The Lord of Uraniborg is a accomplished biography of Tycho Brahe, father of recent astronomy, famed alchemist and littérateur of the sixteenth-century Danish Renaissance. Written in a full of life and interesting variety, Victor Thoren's biography bargains attention-grabbing views on Tycho's existence and offers replacement analyses of almost each point of his medical paintings.

Everywhere and Everywhen: Adventures in Physics and Philosophy

Why does time move and house doesn't? Are there simply 3 dimensions? what's a quantum particle? Nick Huggett exhibits that philosophy -- armed with an influence to investigate basic options and their courting to the human adventure -- has a lot to assert approximately those profound questions on the universe.

Systematicity: The Nature of Science

In Systematicity, Paul Hoyningen-Huene solutions the query "What is technological know-how? " via presenting that clinical wisdom is basically individual from different kinds of data, particularly daily wisdom, by means of being extra systematic. "Science" is the following understood within the broadest attainable experience, encompassing not just the common sciences but additionally arithmetic, the social sciences, and the arts.

Doing Physics, Second Edition: How Physicists Take Hold of the World

Doing Physics makes innovations of physics more uncomplicated to know via bearing on them to daily wisdom. Addressing many of the versions and metaphors that physicists use to provide an explanation for the actual global, Martin H. Krieger describes the conceptual international of physics through analogies to economics, anthropology, theater, carpentry, mechanisms similar to clockworks, and computer device layout.

Additional info for Current Protocols in Cytometry

Example text

When solvent staining is possible for watersoluble fluorochromes, the spectral characteristics can differ significantly from those of fluorochrome in aqueous solution. Surface staining allows many common fluorochromes— especially those used as tags on fluorescent antibodies—to be used for particle staining. , amino group) is covalently bound to a reactive group on the fluorochrome. MESF (molecules of equivalent soluble fluorochrome): measure of particle fluorescence in which the signal from a fluorescent particle is equal to that from a known number of molecules in solution.

Background (noise, fluorescence, scatter): signal present when no particles are flowing in the sample stream. Background noise is one factor that limits the sensitivity of fluorescence detection (see definitions of fluorescence sensitivity and light-scatter sensitivity below). Depending on how low are the signals that one is trying to detect in the sample, different factors are dominant contributors to the background. , lasers turned off), detector noise is the background limit. For photomultiplier tubes (PMTs), the detector background noise is called dark current and is due to random emission of electrons from the photocathode.

A very useful set of definitions that generally cover the field of clinical diagnostics is NCCLS publication NRSCL8-A, Terminology and Definitions for use in NCCLS Documents (NCCLS, 1998a). The definitions for limits below are from the NCCLS definitions. Additional definitions related to quantitative fluorescence cytometry are provided by Henderson et al. (1998). The definitions below should be considered a reasonable point along the way toward authoritative and broadly accepted and understood terminology.

Download PDF sample

Rated 4.99 of 5 – based on 31 votes