Biological Mass Spectrometry by A. L. Burlingame (Eds.)

By A. L. Burlingame (Eds.)

This quantity describes and integrates the recommendations and basics of greater than a decade of progressive advances in either chromatographic and mass spectrometric applied sciences that experience enabled the direct research of biomacromolecules according to se and feature supplied the analytical energy base to herald the recent fields of proteomics and platforms biology.

It additionally covers new biophysical functions corresponding to H/D alternate for examine of conformations, protein-protein and protein-metal and ligand interactions. ultimately it describes atto-to-zepto-mole quantitation of 14C and 3H by means of accelerator mass spectrometry.

*Part 1 of two volumes approximately Mass Spectrometry
*Authoritative and finished remedy of protein mass spectrometry in human mobile biology
*Presents basics, suggestions, instrumentation and bioinformatics
*Provides an summary of proteomics, protein-protein and protein-ligand binding, and biophysical reports

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Mass Spectrom. 32, 135–146. , McCammon, M. , and Robinson, C. V. (2005). ’’ Philos. Trans. R. Soc. A 363, 379–391. , and Jaeger, E. (1992). Peptide sequencing by matrix‐assisted laser‐desorption mass spectrometry. Rapid Comun. Mass Spectrom. 6, 105–108. , Richter, W. , Waterfield, M. , and Burlingame, A. L. (1997). Enhancement of charge remote fragmentation in protonated peptides by high‐energy CID MALDI‐TOF‐MS using ‘‘cold’’ matrices. Int. J. Mass Spectrom. Ion Processes 169/170, 231–240. Sze, S.

At best, this fraction then has the value ([length of packet]/[time between packets]). For example, Pinkston et al. 0025% of the total ion current contributed to the recorded spectra when they interfaced a continuous chemical ionization source to a TOF mass analyzer. Orthogonal Injection. Fortunately TOF instruments can tolerate a relatively large spatial or velocity spread in a plane perpendicular to the spectrometer axis, as illustrated by the large sources (up to $1 cm diameter) typically used in fission fragment desorption (PDMS).

Chem. 75, 1267. Knapp, D. R. (1990). Chemical derivatization for mass spectrometry. Methods Enzymol. 193, 314–329. Krutchinsky, A. , and Chait, B. T. (2001). Automatic identification of proteins with a MALDI-quadrupole ion trap mass spectrometer. Anal. Chem. 73, 5066–5077. Krutchinsky, A. , Loboda, A. , Spicer, V. , and Standing, K. G. (1998). Orthogonal injection of matrix‐assisted laser desorption/ionization ions into a time‐of‐flight spectrometer through a collisional damping interface. J. Am.

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