By Vedpal S. Malik, Erik P. Lillehoj
The applicability of immunotechniques to a wide selection of study difficulties in lots of parts of biology and chemistry has increased dramatically during the last twenty years ever because the creation of monoclonal antibodies and complicated immunosorbent concepts. Exquisitely particular antibody molecules supply technique of separation, quantitative and qualitative research, and localization worthwhile to an individual doing organic or biochemical research.
This useful advisor to immunotechniques is principally designed to be simply understood by means of individuals with little functional adventure utilizing antibodies. It essentially offers targeted, easy-to-follow, step by step equipment for the generally used options that take advantage of the original houses of antibodies and should aid researchers use antibodies to their greatest virtue.
* precise, easy-to-follow, step by step protocols
* handy, easy-to-use format
* huge useful information
* crucial history information
* useful tricks
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Additional resources for Antibody Techniques
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Soluble immune complexes that form in the presence of limiting antibody have unoccupied epitopes on the immunogen. Such complexes may be bound by mlg on specific B cells. B cells express receptors for the Fc region of IgG (FcyR) that are capable of binding the IgG component of the immune complexes. This results in cross-linking of mlg and FcRy, which blocks B cell proliferation (Figure 12). (Muta et al, 1994). The idiotypic determinants of Ig V domains also represent targets for immune regulation.