Analytical Chemistry Progress by Robert B. Green (auth.)

By Robert B. Green (auth.)

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The corresponding amplitudes al depend on the position of the absorption vector ~t, and the emission vector ~t~ within the coordinates of the rotating unit. The rotational relaxation time informs on problems like the sequental flexibility of antibodies, of DNA, of myosin, of membrane proteins or the rotational diffusion of transfer-RNA 47). For example transfer-RNA (ribonucleic acid) which was labelled with the dye ethidium bromide was investigated using nanosecond laser pulses zs,#o). Transfer-RNA molecules participate in protein synthesis according to the genetic code at the ribosomes in the cell.

G. 2). 2). Time resolved observations of the energy transfer in DNA-acridine-orange-complexes were performed by Shapiro et al. 46). They excited the complexes in solution with 10 ps pulses from a mode-locked N d : Y A G laser at 265 nm. The measurement of the fluorescence risetime provides the interval over which the exciton transfer along the DNA takes place. 2) in complexes, where the different mean spacings of the dye molecules along the DNA chain varied, rendered the range of the exciton transfer along the DNA.

For example, fluorescent labelled protein was studied in regard to the surface chemistry of blood 75} Information on transport processes through membranes of red blood cells were obtained by fluorescence investigations using a nitrogen pumped dye laser as excitation source by Marowsky et al. 76}. The membrane potential-dependent uptake and release of dye, which was added to the solution of the blood cells, was observed. The action spectrum of photoreactivation (in yeast cells) was measured by Anders et al.

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